Evaluation of Fresh and Thawed Semen of Pecari tajacu (Artiodactyla: Tayassuidae) in Yucatán Mexico
Journal of Agriculture and Ecology Research International,
Page 1-10
DOI:
10.9734/jaeri/2021/v22i230183
Abstract
This research aimed to characterize the seminal properties of collared peccary Pecari tajacu before and after freezing, and to relate the morphometric measurements of the testes with sperm measurements before freezing. The study design was quasi-experimental in nature. The investigation was carried out at the Xmatkuil Wildlife Management and Conservation Unit and the Centennial Zoo in the state of Yucatan, Mexico, during the months of October to December 2001. Seventeen collared pecaries adult were electroejaculation from two captive populations. Macro and microscopic analyzes of fresh and thawed semen were carried out, also morphometric measurements of the testes, the data of sperm measurements were compared with Student´s t-test, a linear regression model was adjusted between spermatic and morphometric variables of the testis. Logistic regression was performed between seminal freezability and spermatic and morphometric variables. The ejaculate volume was 0.96 ± 0.98 ml, with 62.08 ± 19.10% sperm motility, concentration of 558.61 ± 537.04 x 106 spermatozoa / ml of fresh semen and vigor of 3.7 ± 0.58 on a scale of 1 to 5. On thawing the motility sperm was 35 ± 17.18% and 2.75 ± 0.62 vigor. The measurements of the sperm cell segments did not show a significant difference between the individuals of the two populations. Testicular width and sperm concentration were fitted to the logistic regression model with freezability (1 = yes, 0 = no). It is concluded that testicular width is a factor significantly associated with sperm motility and seminal freezability.
Keywords:
- Cryopreservation
- collared peccary
- reproductive management
- semen
- testicular size
- Peccary tajacu
How to Cite
Rubén, M.-P., Jose, M.-Y., Carlos, S.-M., & Juan, M.-M. (2021). Evaluation of Fresh and Thawed Semen of Pecari tajacu (Artiodactyla: Tayassuidae) in Yucatán Mexico. Journal of Agriculture and Ecology Research International, 22(2), 1-10. https://doi.org/10.9734/jaeri/2021/v22i230183
References
Silva MA, Peixoto GCX, Castelo TS, Lima GL, Silva AM, Oliveira MF, et al. Cryopreservation of collared peccary (Pecari tajacu) semen using different freezing curves, straw sizes, and thawing rates. Cryobiology. 2013;67:50–55. DOI: org/10.1016/j.cryobiol.2013.04.009
Souza PAL, Lima LG, Peixoto XGC, De Souza Castelo T, Oliveira MGC, De Paula VV, et al. Sperm characteristics following freezing in extenders supplemented with whole egg yolk and different concentrations of low-density lipoproteins in the collared peccary (Pecari tajacu). Reprod Biol. 2015;15:223– 228. DOI: 10.1016/j.repbio.2015.10.006.
Silva MA, Peixoto GCX, Lima GL, Bezerra JAB, Campos LB, Paiva ALC, et al. Cryopreservation of collared peccaries (Tayassu tajacu) semen using a powdered coconut water (ACP-116c) based extender plus various concentrations of egg yolk and glycerol. Theriogenology. 2012;78:605–611. DOI:org/10.1016/j.theriogenology.2012.03.006
Bartha MM, Dias HL, Albuquerque NI, Kahwage PR, Guimarães DA, Garcia AR, et al. Microbiological evaluation of fresh semen from captivity kept collared peccary (Pecari tajacu) at Brazilian amazon. Brazilian Journal of Development. 2020;6(9):65920-30.
De la Vega AC, Ruíz R y Wilde RO. Relationship of scrotal circumference with some parameters of seminal quality in criollo goats from the province of Tucumán (Argentina). Relación de la circunferencia escrotal con algunos parámetros de calidad seminal en caprinos criollos de la provincia de Tucumán (Argentina). Zootec Trop. Spanish. 2001;19:455-463. Available:https://tspace.library.utoronto.ca/bitstream/1807/1617/1/zt01026.pdf.
Orellana LR, Espadas MC, Nava MF. Climates. Climas. En: Durán R y Méndez M, editors. Biodiversity and human development. Biodiversidad y Desarrollo Humano. CICY, PPD,-FMAM, CONABIO, SEDUMA. Mérida, Yucatán. Spanish; 2010. Available:http://www.seduma.yucatan.gob.mx/biodiversidad-yucatan/libro-biodiversidad-yucatan.php.
Montes PCR. Breeding the kitam or collared peccary (Pecari tajacu) in a corral. Crianza del kitam o pecarí de collar (Pecari tajacu) en corral. Editorial universidad autónoma de yucatán; mérida yucatán. Spanish; 2005.
Mukul YJM, Ake LJR y montes PRC. Efficiency in obtaining semen through electro-ejaculation in collared peccary (Pecari tajacu) and temazate deer (Mazama pandora. Eficiencia en la obtención de semen mediante electro-‐eyaculación en pecarí de collar (Pecari tajacu) y venado temazate (Mazama pandora). Bio agrociencias. Spanish. 2014;7:15-20. Available:http://www.ccba.uady.mx/revistas/bioagro/V7N2/3.%20Electro-eyaculacion%20en%20pecari.pdf.
Costa DS, Paula TAR. Collection and evaluation of semen of collectors (Tayassu tajacu). Coleta e avaliação do semen de catetos (Tayassu tajacu). Biota Neotrop. Portuguese. 2005;5:1-6. DOI: 10.1590/S1676-06032005000300009 . Statgraphics Technologies, Inc. Statgraphics Plus 5.1. Virginia, USA; 1990-1995.
Ortiz PN. Study of sperm characteristics and cryo perservation in epididymal sperm from Iberian deer obtained postmortem. Estudio de las características espermáticas y de la crioperservación en espermatozoides epididimarios de ciervo ibérico obtenidos postmortem. Tesis doctoral E.T.S. de Ingenieros agrónomos de Albacete. Universidad de Castilla-La Mancha. Albacete España. Spanish; 1999.
Garde JJ, Ortíz N, García AJ, López A, Gallegos L. Cryopreservation of sperm samples collected postmortem and artificial insemination in Iberian deer. Criopreservación postmortem de material espermático e inseminación artificial en el ciervo ibérico. Arch Zootec. Spanish. 1998;47:351-356. Available:https://dialnet.unirioja.es/descarga/articulo/278137.pdf
Garde JJ, Soler AJ, Cassinello J, Crespo C, Malo AF, Espeso G, et al. Sperm cryopreservation in three species of endangered gazelles (Gazella cuvieri, G. dama mhorr and G. dorcas neglecta). Biol Reprod. 2003;69:602-611. DOI: 10.1095/biolreprod.102.012914.
De Segura IAG. Métodos de anestesia, analgesia y eutanasia. Spanish; 2010. Available:https://www.unrc.edu.ar/unrc /coedi/docs/guia-anestesia-eutanasia.pdf.
Kahwage PR, Garcia AR, Guimaraes DAA, Ohashi OM, Luz-Ramos RS, Dias HLT, et al. Testicular biometry, electroejaculation and seminal features of captive collared peccaries (Tayassu tajacu Linnaeus, 1758, Artiodactyla, Tayassuidae) raised in the eastern amazon. Biometria testicular, eletroejaculação e características seminais de caititus, Tayassu tajacu Linnaeus, 1758 (Mammalia, Artiodactyla, Tayassuidae) mantidos em cativeiro na Amazônia Oriental. Acta Amazon. Portuguese. 2010;40(4):771-778. DOI: org/10.1590/S0044-59672010000400019.
Hellgren EC, Lochmiller MS, Amoss JR, Grant WE. Seasonal variation in serum testosterone, testicular measurement and semen characteristics in the collared peccary (Tayassu tajacu). J Reprod Fertil. 1989;85:677-686. DOI: 10.1530/jrf.0.0850677.
Valverde-Abarca A, Madrigal-Valverde M, Solís-Arias J, Paniagua-Madrigal W. Variability of the estimation methods of the boar sperm concentration. Agron. Costarricense. Spanish. 2019;43:25-43.
Bonet OM y Mach N. Nutritional and non-nutritional factors may affect male infertility through epigenetic mechanisms. Factores nutricionales y no nutricionales pueden afectar la fertilidad masculina mediante mecanismos epigenéticos. Nutr Hosp. Spanish. 2016;33(5):1236- 1244. DOI: http://dx.doi.org/10.20960/nh.591
Espinosa-Cervantes Ry, Córdova-Izquierdo A. Effect of heat stress and oxidative stress on sperm function in Mammals. Rev. Complut. Cienc. Vet. Spanish. 2018;12:27-39.
Ribeiro-Peres A, Munita-Barbosa L, Yumi-Kanazawa M, Mello-Martins MI, Ferreira de Souza F. Cryopreservation of bovine spermatozoa from the epididymal tail using conventional and automated methods. Arch Med Vet. 2014;46:31-38.
Maia KM, Souza ALP, Silva AM, Souza-Jr JBF, Costa LLM, Brandao FZ, et al. Environmental effects on collared peccaries (Pecari tajacu) serum testosterone, testicular morphology, and semen quality in the Caatinga biome. Theriogenology. 2019;126:286- 294. DOI:10.1016/j.theriogenology.2018.12.032.
Madrigal-Valverde M, Madrigal-Valverde M. Computer-assisted semen analysis systems in animal reproduction. Agron. Mesoam. 2018;29:469-484.
Abalde GS, Rendal VEM, García GM, López PJM, Carballal RM, Barbero CC, et al. Sperm motility evaluation using two cryoprotectants. Evaluación de la motilidad espermática usando dos crioprotectores. Rev. Iberoam. Fert Rep Hum. Spanish. 2016;33;15-26. Available:http://www.revistafertilidad.org/rif/vplus/arts/226_EVALUACIO__769_N_DE_LA_MOTILIDAD.pdf
Castelo TS, FSB Bezerra ALP, Souza MAP, Moreira VV, Paula MF, Oliveira AR Silva. Influence of the thawing rate on the cryopreservation of semen from collared peccaries (Tayassu tajacu) using tris-based extenders. Theriogenology. 2010;74:1060–1065. DOI:10.1016/j.theriogenology.2010.05.002.
Sousa PC, Santos EAA, Souza ALP, Lima GL, Barros FFPC, Oliveira M.F, et al. Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu). Pesq. Vet. Bras. 2013;33(7):924-930. DOI: http://dx.doi.org/10.1590/S0100-736X2013000700014.
Sowls LK. Javelinas and other peccaries, their biology, management and use. Second ed. Texas A & M University Press. Tucson, Arizona; 1997.
Sonner JB, Miglino MA, Dos Santos TC, Carvalhal R, Neto AC, Moura CB, et al. Macroscopic and morphometric features of testicles in collared peccary and white-lipped peccary. Aspectos macroscópicos e morfométricos dos testículos en catetos e queixadas. Biota Neotrop. Portuguese. 2004;4:1-13. DOI: https://doi.org/10.1590/S1676-06032004000200006.
Peixoto GCX, Silva MA, Castelo TS, Silva AM, Bezerra JAB, Souza ALP, Oliveira MF, et al. Individual variation related to testicular biometry and semen characteristics in collared peccaries (Tayassu Tajacu Linnaeus, 1758). Anim Reprod Sci 2012;134:191-196. DOI: 10.1016/j.anireprosci.2012.08.026.
Clark SG, Schaeffer DJ, Althouse GC. B-Mode ultrasonographic evaluation of paired testicular diameter of mature boars in relation to average total sperm numbers. Theriogenology.2003;60:1011-1023. DOI: 10.1016/s0093-691x(03)00127-4.
Ortega CM y Aurelio Cayuela DA. Uconditioned logistic regression and sample size: A reference source review. Regresión logística no condicionada y tamaño de muestra: Una revisión bibliográfica. Rev Esp Salud Publica. Spanish. 2002;76:85-93. Available:http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1135-57272002000200002.
Statgraphics. Net. sin año. Tutoriales. Recursos. logistic regression. Regresión Logística. Spanish. Available:https://statgraphics.net/download/855/
Ferrugem MJC. Examination of reproductive health and fertility disorders of bulls. In: Galina C and valencia J editors. Reproduction of domestic animals. Examen de la salud reproductiva y alteraciones de la fertilidad de los toros. En: Galina C y Valencia J editores. Reproducción de animales domésticos. Limusa Noriega segunda edición. México DF. Spanish; 2006.
Pimentel GJR, Perezgrovas GL, Zaragoza My, Rodríguez GG. Integral reproductive characterization of rams in Chiapas wool-sheep. Caracterización reproductiva integral del morueco en el ganado lanar de Chiapas. Arch Zootec; Spanish. 2005;54:557-564.
Souza PAL, Lima LG, Peixoto XGC, De Souza Castelo T, Oliveira MGC, De Paula VV, et al. Sperm characteristics following freezing in extenders supplemented with whole egg yolk and different concentrations of low-density lipoproteins in the collared peccary (Pecari tajacu). Reprod Biol. 2015;15:223– 228. DOI: 10.1016/j.repbio.2015.10.006.
Silva MA, Peixoto GCX, Lima GL, Bezerra JAB, Campos LB, Paiva ALC, et al. Cryopreservation of collared peccaries (Tayassu tajacu) semen using a powdered coconut water (ACP-116c) based extender plus various concentrations of egg yolk and glycerol. Theriogenology. 2012;78:605–611. DOI:org/10.1016/j.theriogenology.2012.03.006
Bartha MM, Dias HL, Albuquerque NI, Kahwage PR, Guimarães DA, Garcia AR, et al. Microbiological evaluation of fresh semen from captivity kept collared peccary (Pecari tajacu) at Brazilian amazon. Brazilian Journal of Development. 2020;6(9):65920-30.
De la Vega AC, Ruíz R y Wilde RO. Relationship of scrotal circumference with some parameters of seminal quality in criollo goats from the province of Tucumán (Argentina). Relación de la circunferencia escrotal con algunos parámetros de calidad seminal en caprinos criollos de la provincia de Tucumán (Argentina). Zootec Trop. Spanish. 2001;19:455-463. Available:https://tspace.library.utoronto.ca/bitstream/1807/1617/1/zt01026.pdf.
Orellana LR, Espadas MC, Nava MF. Climates. Climas. En: Durán R y Méndez M, editors. Biodiversity and human development. Biodiversidad y Desarrollo Humano. CICY, PPD,-FMAM, CONABIO, SEDUMA. Mérida, Yucatán. Spanish; 2010. Available:http://www.seduma.yucatan.gob.mx/biodiversidad-yucatan/libro-biodiversidad-yucatan.php.
Montes PCR. Breeding the kitam or collared peccary (Pecari tajacu) in a corral. Crianza del kitam o pecarí de collar (Pecari tajacu) en corral. Editorial universidad autónoma de yucatán; mérida yucatán. Spanish; 2005.
Mukul YJM, Ake LJR y montes PRC. Efficiency in obtaining semen through electro-ejaculation in collared peccary (Pecari tajacu) and temazate deer (Mazama pandora. Eficiencia en la obtención de semen mediante electro-‐eyaculación en pecarí de collar (Pecari tajacu) y venado temazate (Mazama pandora). Bio agrociencias. Spanish. 2014;7:15-20. Available:http://www.ccba.uady.mx/revistas/bioagro/V7N2/3.%20Electro-eyaculacion%20en%20pecari.pdf.
Costa DS, Paula TAR. Collection and evaluation of semen of collectors (Tayassu tajacu). Coleta e avaliação do semen de catetos (Tayassu tajacu). Biota Neotrop. Portuguese. 2005;5:1-6. DOI: 10.1590/S1676-06032005000300009 . Statgraphics Technologies, Inc. Statgraphics Plus 5.1. Virginia, USA; 1990-1995.
Ortiz PN. Study of sperm characteristics and cryo perservation in epididymal sperm from Iberian deer obtained postmortem. Estudio de las características espermáticas y de la crioperservación en espermatozoides epididimarios de ciervo ibérico obtenidos postmortem. Tesis doctoral E.T.S. de Ingenieros agrónomos de Albacete. Universidad de Castilla-La Mancha. Albacete España. Spanish; 1999.
Garde JJ, Ortíz N, García AJ, López A, Gallegos L. Cryopreservation of sperm samples collected postmortem and artificial insemination in Iberian deer. Criopreservación postmortem de material espermático e inseminación artificial en el ciervo ibérico. Arch Zootec. Spanish. 1998;47:351-356. Available:https://dialnet.unirioja.es/descarga/articulo/278137.pdf
Garde JJ, Soler AJ, Cassinello J, Crespo C, Malo AF, Espeso G, et al. Sperm cryopreservation in three species of endangered gazelles (Gazella cuvieri, G. dama mhorr and G. dorcas neglecta). Biol Reprod. 2003;69:602-611. DOI: 10.1095/biolreprod.102.012914.
De Segura IAG. Métodos de anestesia, analgesia y eutanasia. Spanish; 2010. Available:https://www.unrc.edu.ar/unrc /coedi/docs/guia-anestesia-eutanasia.pdf.
Kahwage PR, Garcia AR, Guimaraes DAA, Ohashi OM, Luz-Ramos RS, Dias HLT, et al. Testicular biometry, electroejaculation and seminal features of captive collared peccaries (Tayassu tajacu Linnaeus, 1758, Artiodactyla, Tayassuidae) raised in the eastern amazon. Biometria testicular, eletroejaculação e características seminais de caititus, Tayassu tajacu Linnaeus, 1758 (Mammalia, Artiodactyla, Tayassuidae) mantidos em cativeiro na Amazônia Oriental. Acta Amazon. Portuguese. 2010;40(4):771-778. DOI: org/10.1590/S0044-59672010000400019.
Hellgren EC, Lochmiller MS, Amoss JR, Grant WE. Seasonal variation in serum testosterone, testicular measurement and semen characteristics in the collared peccary (Tayassu tajacu). J Reprod Fertil. 1989;85:677-686. DOI: 10.1530/jrf.0.0850677.
Valverde-Abarca A, Madrigal-Valverde M, Solís-Arias J, Paniagua-Madrigal W. Variability of the estimation methods of the boar sperm concentration. Agron. Costarricense. Spanish. 2019;43:25-43.
Bonet OM y Mach N. Nutritional and non-nutritional factors may affect male infertility through epigenetic mechanisms. Factores nutricionales y no nutricionales pueden afectar la fertilidad masculina mediante mecanismos epigenéticos. Nutr Hosp. Spanish. 2016;33(5):1236- 1244. DOI: http://dx.doi.org/10.20960/nh.591
Espinosa-Cervantes Ry, Córdova-Izquierdo A. Effect of heat stress and oxidative stress on sperm function in Mammals. Rev. Complut. Cienc. Vet. Spanish. 2018;12:27-39.
Ribeiro-Peres A, Munita-Barbosa L, Yumi-Kanazawa M, Mello-Martins MI, Ferreira de Souza F. Cryopreservation of bovine spermatozoa from the epididymal tail using conventional and automated methods. Arch Med Vet. 2014;46:31-38.
Maia KM, Souza ALP, Silva AM, Souza-Jr JBF, Costa LLM, Brandao FZ, et al. Environmental effects on collared peccaries (Pecari tajacu) serum testosterone, testicular morphology, and semen quality in the Caatinga biome. Theriogenology. 2019;126:286- 294. DOI:10.1016/j.theriogenology.2018.12.032.
Madrigal-Valverde M, Madrigal-Valverde M. Computer-assisted semen analysis systems in animal reproduction. Agron. Mesoam. 2018;29:469-484.
Abalde GS, Rendal VEM, García GM, López PJM, Carballal RM, Barbero CC, et al. Sperm motility evaluation using two cryoprotectants. Evaluación de la motilidad espermática usando dos crioprotectores. Rev. Iberoam. Fert Rep Hum. Spanish. 2016;33;15-26. Available:http://www.revistafertilidad.org/rif/vplus/arts/226_EVALUACIO__769_N_DE_LA_MOTILIDAD.pdf
Castelo TS, FSB Bezerra ALP, Souza MAP, Moreira VV, Paula MF, Oliveira AR Silva. Influence of the thawing rate on the cryopreservation of semen from collared peccaries (Tayassu tajacu) using tris-based extenders. Theriogenology. 2010;74:1060–1065. DOI:10.1016/j.theriogenology.2010.05.002.
Sousa PC, Santos EAA, Souza ALP, Lima GL, Barros FFPC, Oliveira M.F, et al. Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu). Pesq. Vet. Bras. 2013;33(7):924-930. DOI: http://dx.doi.org/10.1590/S0100-736X2013000700014.
Sowls LK. Javelinas and other peccaries, their biology, management and use. Second ed. Texas A & M University Press. Tucson, Arizona; 1997.
Sonner JB, Miglino MA, Dos Santos TC, Carvalhal R, Neto AC, Moura CB, et al. Macroscopic and morphometric features of testicles in collared peccary and white-lipped peccary. Aspectos macroscópicos e morfométricos dos testículos en catetos e queixadas. Biota Neotrop. Portuguese. 2004;4:1-13. DOI: https://doi.org/10.1590/S1676-06032004000200006.
Peixoto GCX, Silva MA, Castelo TS, Silva AM, Bezerra JAB, Souza ALP, Oliveira MF, et al. Individual variation related to testicular biometry and semen characteristics in collared peccaries (Tayassu Tajacu Linnaeus, 1758). Anim Reprod Sci 2012;134:191-196. DOI: 10.1016/j.anireprosci.2012.08.026.
Clark SG, Schaeffer DJ, Althouse GC. B-Mode ultrasonographic evaluation of paired testicular diameter of mature boars in relation to average total sperm numbers. Theriogenology.2003;60:1011-1023. DOI: 10.1016/s0093-691x(03)00127-4.
Ortega CM y Aurelio Cayuela DA. Uconditioned logistic regression and sample size: A reference source review. Regresión logística no condicionada y tamaño de muestra: Una revisión bibliográfica. Rev Esp Salud Publica. Spanish. 2002;76:85-93. Available:http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1135-57272002000200002.
Statgraphics. Net. sin año. Tutoriales. Recursos. logistic regression. Regresión Logística. Spanish. Available:https://statgraphics.net/download/855/
Ferrugem MJC. Examination of reproductive health and fertility disorders of bulls. In: Galina C and valencia J editors. Reproduction of domestic animals. Examen de la salud reproductiva y alteraciones de la fertilidad de los toros. En: Galina C y Valencia J editores. Reproducción de animales domésticos. Limusa Noriega segunda edición. México DF. Spanish; 2006.
Pimentel GJR, Perezgrovas GL, Zaragoza My, Rodríguez GG. Integral reproductive characterization of rams in Chiapas wool-sheep. Caracterización reproductiva integral del morueco en el ganado lanar de Chiapas. Arch Zootec; Spanish. 2005;54:557-564.
-
Abstract View: 52 times
PDF Download: 31 times
Download Statistics
Downloads
Download data is not yet available.
